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dc.contributor.authorDavinna L., Ligons
dc.contributor.authorTuncer, Ceren
dc.contributor.authorLinowes, Brett A.
dc.contributor.authorAkcay, Izzet Mehmet
dc.contributor.authorBelkis Atasever, Arslan
dc.contributor.authorCevik, Safak Isil
dc.contributor.authorKeller, Hilary R.
dc.contributor.authorLuckey, Megan A.
dc.contributor.authorFeigenbaum, Lionel
dc.contributor.authorMoroy, Tarik
dc.contributor.authorErsahin, Tulin
dc.contributor.authorRengul, Atalay
dc.contributor.authorErman, Batu
dc.contributor.authorPark, Jung Hyun
dc.date.accessioned2014-09-23T08:15:01Z
dc.date.available2014-09-23T08:15:01Z
dc.date.issued2012
dc.identifier.citationLigons DL, Tuncer C, Linowes BA, Akcay IM, Kurtulus S, Deniz E, Atasever Arslan B, Cevik SI, Keller HR, Luckey M, Feigenbaum L, Moroy T, Ersahin T, Atalay R, Erman B, Park JH. Transcriptional Control of Interleukin-7 Receptor Expression by Growth Factor Independence-1. J Biol Chem 2012; 287(41):34386-99.tr_TR
dc.identifier.urihttp://earsiv.uskudar.edu.tr/xmlui/handle/123456789/214
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/pubmed/22865857
dc.description.abstractInterleukin-7 receptor α (IL-7Rα) is essential for T cell survival and differentiation. Glucocorticoids are potent enhancers of IL-7Rα expression with diverse roles in T cell biology. Here we identify the transcriptional repressor, growth factor independent-1 (Gfi1), as a novel intermediary in glucocorticoid-induced IL-7Rα up-regulation. We found Gfi1 to be a major inhibitory target of dexamethasone by microarray expression profiling of 3B4.15 T-hybridoma cells. Concordantly, retroviral transduction of Gfi1 significantly blunted IL-7Rα up-regulation by dexamethasone. To further assess the role of Gfi1 in vivo, we generated bacterial artificial chromosome (BAC) transgenic mice, in which a modified Il7r locus expresses GFP to report Il7r gene transcription. By introducing this BAC reporter transgene into either Gfi1-deficient or Gfi1-transgenic mice, we document in vivo that IL-7Rα transcription is up-regulated in the absence of Gfi1 and down-regulated when Gfi1 is overexpressed. Strikingly, the in vivo regulatory role of Gfi1 was specific for CD8(+), and not CD4(+) T cells or immature thymocytes. These results identify Gfi1 as a specific transcriptional repressor of the Il7r gene in CD8 T lymphocytes in vivo.tr_TR
dc.language.isoengtr_TR
dc.relation.ispartofseriesSCI;
dc.relation.isversionof10.1074/jbc.M112.378687tr_TR
dc.subjectGlucocorticoidstr_TR
dc.subjectImmunologytr_TR
dc.subjectThymocytetr_TR
dc.subjectTranscriptiontr_TR
dc.subjectRepressortr_TR
dc.subjectZinc Fingertr_TR
dc.titleCD8 lineage-specific regulation of interleukin-7 receptor expression by the transcriptional repressor Gfi1.tr_TR
dc.typeArticletr_TR
dc.relation.journalJournal of Biological Chemistrytr_TR
dc.contributor.departmentÜsküdar Üniversitesi, Mühendislik Fakültesi, Moleküler Biyoloji ve Genetiktr_TR
dc.contributor.authorIDTR162159tr_TR


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